FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n02_ALm222.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n02_ALm222.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16380564
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA16400.012.1570369
GGGGGGA143400.010.1929952
CGGGGAT52750.010.0763621
CGCGGGT33100.09.6062751
CTACCGT21900.09.5368938
CGCGATT6102.1325739E-48.5579761
CGGGGTT53400.08.4428791
GTCGGAT17500.08.1356331
CGGGTAT17250.07.97842361
CGACCTA9551.0065305E-67.95154144
GCGTCGT140000.07.943835794-95
CGCATAT10303.760124E-77.83285141
CGGGAAT36400.07.82272531
CGGCGAT28350.07.7003941
CCCGTAT24700.07.6854841
CGAGAAT34000.07.67700771
AGATCGG368400.07.52127394-95
AAGGGGG141500.07.507948480-81
AGCGTCG162800.07.488164494-95
GATCGGA330450.07.44298394-95
Produced by FastQC (version 0.11.5)