FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n02_ALm221.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n02_ALm221.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15429219
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA16650.014.2557259
CTACCGT21050.011.0495648
CGCGTAT3700.002386943710.2617261
CGCGGGT28100.09.96501351
GGGGGGA127300.09.6185142
ACTACCG28250.08.905137
CGGGGAT49250.08.8657151
CGCGGTT17600.08.629181
CCGTATG18900.08.28887510-11
CGCGAAT8107.718385E-68.2030481
CTCGTAT13953.274181E-118.1652451
CCGGGAT43750.08.1360831
CGTATGG16700.08.10193412-13
CGGGATT26000.07.8492351
CGTATCG16401.8189894E-127.8133633
GCGTCGT121250.07.66303594-95
AGCGTCG138350.07.64340194-95
ACCGTAT18150.07.58516110-11
CGACGGA11906.002483E-87.57772541
CGCCAAT27300.07.47546151
Produced by FastQC (version 0.11.5)