FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n02_ALm220.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n02_ALm220.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15622982
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT15350.010.8210261
CGCGGGT31800.010.2974761
CGGGGAT52150.09.5552881
GGGGGGA128450.09.4213282
TACCGTA15900.08.9571089
CGCGAAT9551.1781594E-88.944951
GTCGATT12854.7293724E-118.4944151
CCGGGAT43550.08.28198051
CTCGGAT43450.07.97336961
GGGGAAT83050.07.8287011
CCCGTAT24950.07.79869841
CGGGATT26650.07.6573771
CGGGTAT17055.456968E-127.5153321
CGGGGTT54550.07.481911
GCGTCGT120600.07.3891694-95
GATCGGA274150.07.36786794-95
CGGTAAT16201.10958354E-107.3237551
AGCGTCG138650.07.318450594-95
AGATCGG309350.07.297676694-95
TAGATCG52650.07.266723694-95
Produced by FastQC (version 0.11.5)