FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n02_ALm219.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n02_ALm219.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16283663
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT32350.011.2965461
GGGGGGA134500.09.80972
GTCCGAT14850.09.2683151
TACCGTA16250.08.1797119
CGCGATT7001.05065636E-48.1360271
CGGGGTT56600.08.1336321
TCGGATT26650.08.0139862
CGATTGG16600.08.00591854
CCGGTAT20250.07.96862031
GCGGGAT35250.07.80904871
CCGGGAT46450.07.6631051
GGGGAAT84650.07.56895641
CTCGGTT29750.07.4979081
CGGGGAT52850.07.4535351
GCGTCGT134650.07.359464694-95
GATCGGA304500.07.289137494-95
CGGGATT29350.07.27675252
CGGGTAT17701.4551915E-117.2396861
CCGCAAT24450.07.18211031
AGCGTCG153000.07.12915194-95
Produced by FastQC (version 0.11.5)