FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n02_ALm218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n02_ALm218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17386108
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA15750.011.1523359
CGCGGGT34300.011.0693741
CTACCGT21200.09.852338
CCGGGAT49050.09.0952791
CGGGGAT56050.08.2134061
CGATTGG16250.08.1782774
CGGGAAT40800.08.0263141
CTCGGAT49350.07.88594771
AAAGGGG155850.07.730770678-79
GTCGATT13601.3424142E-97.6773431
GTCCGAT15504.0017767E-117.6548291
CGGGGTT58600.07.6130271
CGGGTAT18150.07.58313041
GGGGAAT85750.07.5825211
ACTACCG27850.07.32915937
AAGGGGG151750.07.141236380-81
CGGATCG11401.8758892E-67.0775633
CGGGCAT75600.07.0311021
CGCCAAT35300.06.99126631
CCGCAAT27350.06.94112441
Produced by FastQC (version 0.11.5)