FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n02_ALm217.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n02_ALm217.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17136351
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA174300.015.3298172
CGCGGGT34450.013.088031
AAGGGGG172200.011.09611180-81
GGGGGAG215250.010.2087813
AAAAGGG200600.010.09338678-79
AGAGCGG67450.09.3602928
CGGGGTT53850.09.2542971
TAGGGGG61800.09.18417450-51
GGGGAGA196500.08.7195634
CGGGGAT55850.08.4981
CTCGGAT47550.08.4841491
GAGCGGG108150.08.4279819
TACCGTA19200.08.406729
TCGGATT28200.08.2465612
GAGAGCG81250.08.1206997
CCGGAAT39800.07.9897211
GCGGGTC58900.07.97711472
GTCGGAT17700.07.77614931
AGGGGGG207050.07.66755282-83
CGGGAAT41500.07.5480661
Produced by FastQC (version 0.11.5)