FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36179879
Sequences flagged as poor quality0
Sequence length101
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1157444931.991397760064373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA20550.023.99456836-37
GCGAAAT24350.020.93152636-37
AGAGCAC116400.020.3974118
TCCGCGA23000.019.89156534-35
CCGCGAA25000.019.4380834-35
AGGGGGG99700.019.210861
TCTCGTA41350.017.44287742-43
CGTCTGA69050.017.36688216-17
CTCGTAT39250.017.11234944-45
ACACGTC67800.016.98590712-13
ACGTCTG68200.016.78348514-15
CACGTCT70050.016.74620814-15
ACTCCGC26500.016.2813132-33
CACACGT73800.016.11868312-13
TCGGAAG161750.015.7346653
CGGAAGA165550.015.688564
ATGCCGT53750.015.59609348-49
ATCGGAA165050.015.4786532
CCGGCTA22650.015.07155632-33
GATCGGA166950.014.8885431
Produced by FastQC (version 0.11.5)