FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n01_ALm232.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n01_ALm232.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14679348
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT647090.44081658122690465TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA88350.041.0474636-37
CGTATGC90050.040.0353246-47
TCGTATG88500.039.7437244-45
CTCGTAT87800.038.89744644-45
TATGCCG96750.037.31232548-49
CCGCGAA100000.035.9804434-35
TCCGCGA101850.035.9331634-35
TCTCGTA96650.034.4513242-43
GCGAAAT104750.033.82739636-37
CGAAATC104150.033.15592238-39
ATCTCGT102700.032.95369342-43
ACTCCGC114800.031.71422232-33
ATGCCGT112850.031.48398848-49
GTATGCC119250.030.13254546-47
GCCGTCT115100.030.12580950-51
AATCTCG113150.029.57405940-41
CTCCGCG123850.029.2625632-33
TGCCGTC124200.029.02761750-51
CCGTCTT122850.028.92138552-53
CACTCCG131000.027.48411230-31
Produced by FastQC (version 0.11.5)