FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n01_ALm231.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n01_ALm231.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16043662
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT822440.5126261074310839TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC111550.041.4101746-47
CGCGAAA112700.041.21929636-37
TCGTATG109700.041.00426544-45
CTCGTAT108700.040.22350344-45
TATGCCG120500.038.3741448-49
CCGCGAA123500.037.1720534-35
TCTCGTA115800.036.81393442-43
TCCGCGA128250.036.7026934-35
GCGAAAT126150.035.93969336-37
ATCTCGT124900.034.53104842-43
AATCTCG125600.033.84712240-41
ACTCCGC142700.032.98612632-33
CGAAATC133450.032.8171438-39
GTATGCC140150.032.6038646-47
ATGCCGT140300.032.3322148-49
CCGTCTT144900.031.58452852-53
GCCGTCT144150.030.99097450-51
TGCCGTC149500.030.7715650-51
CTCCGCG152850.030.22078732-33
CACTCCG156000.029.6714130-31
Produced by FastQC (version 0.11.5)