FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n01_ALm230.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n01_ALm230.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15816225
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT446990.2826148464630467TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA65850.036.67909236-37
TCGTATG66450.035.7765744-45
TATGCCG67950.035.4066748-49
CGTATGC69250.034.77601646-47
CTCGTAT69200.033.42815844-45
GCGAAAT77100.030.89583436-37
CCGCGAA78600.030.78942534-35
CGAAATC79550.029.19811238-39
AATCTCG78350.028.67545740-41
TCCGCGA85400.028.64371334-35
TCTCGTA79200.028.57774742-43
ATCTCGT85250.026.71680542-43
ACTCCGC93350.026.40797232-33
ATGCCGT99300.024.1088448-49
CACTCCG101200.023.81988130-31
GTATGCC102800.023.40334946-47
CCGTCTT101500.023.35229552-53
CTCCGCG104450.023.3514632-33
GCCGTCT104500.022.86367850-51
TGCCGTC115000.021.16851650-51
Produced by FastQC (version 0.11.5)