FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n01_ALm229.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n01_ALm229.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15813535
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT441800.27938092273485976TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC68600.037.04344646-47
TATGCCG69950.036.56711248-49
TCGTATG68350.036.2060344-45
CGCGAAA70100.036.14898336-37
CTCGTAT68950.034.89208244-45
GCGAAAT81150.031.08033436-37
CCGCGAA84000.030.13872134-35
TCCGCGA85750.030.0221734-35
TCTCGTA81400.029.08885442-43
CGAAATC83800.028.8225338-39
AATCTCG82850.028.57979840-41
ATCTCGT86150.027.67797342-43
ACTCCGC96200.026.76105332-33
ATGCCGT100150.025.37438448-49
CACTCCG105350.024.23395330-31
GTATGCC105850.024.16442946-47
CCGTCTT109850.023.24194752-53
GCCGTCT108100.023.22273650-51
CTCCGCG110150.023.04847332-33
TGCCGTC121800.021.2151350-51
Produced by FastQC (version 0.11.5)