FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n01_ALm228.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n01_ALm228.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16220064
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT534610.32959795966279787TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG79350.037.5023844-45
CGCGAAA80950.037.024936-37
TATGCCG81700.036.8895248-49
CGTATGC82000.036.724846-47
CTCGTAT81750.035.47175244-45
CCGCGAA92400.032.15402634-35
GCGAAAT92550.031.99936736-37
TCTCGTA90050.031.6221142-43
CGAAATC94050.030.75684738-39
TCCGCGA98000.030.704434-35
AATCTCG94450.029.9728740-41
ATCTCGT97900.029.4504142-43
ACTCCGC108350.027.90295432-33
ATGCCGT112550.026.52486648-49
GTATGCC114900.026.22983746-47
CACTCCG118000.025.5807730-31
CCGTCTT119450.024.95286252-53
CTCCGCG120400.024.93281432-33
GCCGTCT118950.024.73828750-51
TGCCGTC130650.023.10462450-51
Produced by FastQC (version 0.11.5)