FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n01_ALm227.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n01_ALm227.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15832015
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT459080.28996940692640827TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC69000.037.03571346-47
TCGTATG67750.036.59709544-45
CGCGAAA69800.036.30477536-37
CTCGTAT68050.035.73775544-45
TATGCCG71150.035.7164648-49
GCGAAAT80450.031.02640336-37
TCTCGTA77600.030.75826542-43
CGAAATC80750.030.35251238-39
CCGCGAA83650.030.18014734-35
TCCGCGA89050.028.8834234-35
AATCTCG83000.028.7284240-41
ATCTCGT89000.027.40551242-43
ACTCCGC97700.026.5205732-33
ATGCCGT99100.025.49925848-49
GTATGCC101600.024.98857346-47
CACTCCG104550.024.10142330-31
GCCGTCT105400.023.54694450-51
CCGTCTT107850.023.38646552-53
CTCCGCG113150.022.68944432-33
TGCCGTC117950.021.58518250-51
Produced by FastQC (version 0.11.5)