FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n01_ALm226.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n01_ALm226.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15709930
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT652670.41545060990087157TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC93450.038.93474646-47
TATGCCG94500.038.55270848-49
TCGTATG92750.038.46028544-45
CGCGAAA95750.038.09798436-37
CTCGTAT91300.037.66641244-45
CCGCGAA107150.034.0225234-35
GCGAAAT107500.033.42566336-37
TCTCGTA102550.033.1637142-43
TCCGCGA112650.032.82522234-35
AATCTCG103250.032.75464640-41
CGAAATC106850.032.47326738-39
ATCTCGT109900.031.01058842-43
ACTCCGC123000.030.2177132-33
ATGCCGT122750.029.6413948-49
CCGTCTT127600.028.29129652-53
GTATGCC129650.028.08196646-47
CACTCCG131300.028.05434430-31
CTCCGCG134900.027.35844432-33
GCCGTCT131000.027.26685750-51
TGCCGTC142600.025.69833250-51
Produced by FastQC (version 0.11.5)