FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n01_ALm225.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n01_ALm225.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16788775
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT646310.3849655498986674TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG89400.038.12158244-45
CGTATGC91150.037.91090446-47
TATGCCG92400.037.29528448-49
CGCGAAA93450.037.07899536-37
CTCGTAT89100.036.5173644-45
GCGAAAT105550.032.69334436-37
TCTCGTA99700.032.6826642-43
CCGCGAA106500.032.6690734-35
TCCGCGA109800.031.90350734-35
CGAAATC104750.031.65108138-39
AATCTCG103450.031.4061540-41
ATCTCGT109700.029.66008642-43
ATGCCGT121300.028.5858148-49
ACTCCGC123400.028.52216132-33
GTATGCC126500.027.71109246-47
CCGTCTT126550.026.9686352-53
GCCGTCT126500.026.94137850-51
CTCCGCG132500.026.41989132-33
CACTCCG134250.026.2346330-31
TGCCGTC140600.024.72943750-51
Produced by FastQC (version 0.11.5)