FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n01_ALm224.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n01_ALm224.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15027809
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT287850.1915448885462944TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG52300.034.28465344-45
CGTATGC53500.034.0486346-47
TATGCCG55500.032.90773448-49
CTCGTAT53300.032.61657344-45
TCTCGTA62150.026.63464242-43
ATCTCGT69250.023.56091742-43
CGGCTAT80350.022.9659234-35
CCGGCTA80400.022.47905532-33
ATGCCGT85300.021.16065848-49
GTATGCC86250.021.12002246-47
TCACCGG88700.020.91113330-31
TATGATC80800.020.51617438-39
ACCGGCT91400.020.42329232-33
CCGTCTT90050.020.41360352-53
GCCGTCT89400.020.1104750-51
GTCACCG92900.019.60787628-29
TGCCGTC101950.018.38034450-51
GATCTCG98100.016.75282140-41
CGTCTTC109700.016.7137252-53
GCTATGA118950.015.69305636-37
Produced by FastQC (version 0.11.5)