FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n01_ALm223.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n01_ALm223.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16082616
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT347080.21581066164857754TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC62500.034.7697846-47
TATGCCG64000.034.02950348-49
TCGTATG63300.033.76755544-45
CTCGTAT62950.032.7102744-45
TCTCGTA73950.026.62415342-43
ATCTCGT78150.024.95017642-43
CGGCTAT90900.024.0110134-35
ATGCCGT92100.023.64699248-49
CCGGCTA91450.023.60697232-33
GTATGCC96450.022.75257746-47
GCCGTCT97550.022.47187250-51
ACCGGCT104200.021.06029132-33
TATGATC94750.020.97994438-39
TCACCGG105650.020.68126730-31
GTCACCG103550.020.596128-29
TGCCGTC109850.020.085450-51
CCGTCTT110400.019.89919552-53
GATCTCG108300.017.96029940-41
CGTCTTC132450.016.53262552-53
GCTATGA135750.016.32292236-37
Produced by FastQC (version 0.11.5)