FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n01_ALm222.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n01_ALm222.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16380564
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT375670.22933886769710737TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG66100.035.96584748-49
CGTATGC67500.035.67696846-47
TCGTATG66450.035.27588344-45
CTCGTAT67050.033.68506644-45
TCTCGTA76600.028.0594342-43
ATCTCGT79850.026.82814642-43
CGGCTAT94950.025.13746834-35
ATGCCGT95550.024.93032648-49
CCGGCTA98150.023.83400532-33
GTATGCC100450.023.61942346-47
CCGTCTT103900.023.15529352-53
TCACCGG107000.022.61744130-31
GCCGTCT105950.022.50559250-51
ACCGGCT106600.022.50169632-33
TATGATC101600.021.10822938-39
GTCACCG112200.020.99767528-29
TGCCGTC117800.020.6448850-51
GATCTCG113950.018.79965640-41
CGTCTTC127550.018.78741652-53
GCTATGA134550.017.68624936-37
Produced by FastQC (version 0.11.5)