FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n01_ALm221.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n01_ALm221.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15429219
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT339440.2199981735951768TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC54900.034.69423746-47
TCGTATG53550.034.41588644-45
TATGCCG56450.033.70007748-49
CTCGTAT54700.032.5634744-45
TCTCGTA63200.026.56818642-43
ATCTCGT69700.024.05644442-43
CGGCTAT83200.022.83606334-35
CCGGCTA82650.022.44206432-33
ATGCCGT87100.021.73214148-49
TCACCGG89550.021.50866330-31
ACCGGCT91100.021.16860632-33
GTATGCC89100.021.00408746-47
GCCGTCT92750.020.28032950-51
CCGTCTT94500.020.20635452-53
GTCACCG97500.019.17023828-29
TATGATC89250.018.9199338-39
TGCCGTC105300.018.31434650-51
GATCTCG99550.016.84302540-41
CGTCTTC114200.016.57509252-53
CACCGGC120700.015.83969230-31
Produced by FastQC (version 0.11.5)