FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n01_ALm220.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n01_ALm220.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15622982
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT320840.20536412318723787TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG52700.035.28651444-45
CGTATGC54300.034.77162646-47
TATGCCG55800.033.66708448-49
CTCGTAT56950.031.56892644-45
TCTCGTA66650.025.44234542-43
CGGCTAT79550.023.79430434-35
ATCTCGT72950.023.1474642-43
CCGGCTA83100.022.6349632-33
GTATGCC86950.021.63282446-47
TCACCGG91200.021.0413830-31
ATGCCGT90100.021.0349448-49
ACCGGCT92200.020.76154732-33
GCCGTCT93700.020.07468650-51
CCGTCTT95150.019.91853352-53
TATGATC87050.019.86181338-39
GTCACCG99400.019.09041228-29
TGCCGTC107250.017.98133550-51
GATCTCG105000.016.28551740-41
CGTCTTC117300.016.07628652-53
GCTATGA119250.015.85293136-37
Produced by FastQC (version 0.11.5)