FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n01_ALm219.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n01_ALm219.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16283663
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT354080.2174449323840711TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC60100.034.142646-47
TATGCCG61200.033.52913748-49
TCGTATG59700.033.4164544-45
CTCGTAT61100.032.06772244-45
TCTCGTA72000.025.43192542-43
CGGCTAT87900.023.47934-35
ATCTCGT78200.023.41558542-43
CCGGCTA87250.022.89183432-33
ATGCCGT90500.022.30644648-49
GTATGCC92300.022.10287946-47
ACCGGCT98950.020.9291132-33
TCACCGG100600.020.63308330-31
CCGTCTT101400.020.37705252-53
GCCGTCT100400.020.15421750-51
TATGATC93250.020.01835638-39
GTCACCG108150.018.5778128-29
TGCCGTC116600.017.70032750-51
GATCTCG108150.016.82127640-41
CGTCTTC125800.016.44362452-53
GCTATGA124200.016.2918636-37
Produced by FastQC (version 0.11.5)