FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n01_ALm218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n01_ALm218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17386108
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT476370.27399461685156906TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG72400.036.41186544-45
CGTATGC73350.036.26369546-47
TATGCCG74750.035.6800848-49
CTCGTAT72350.034.00789344-45
TCTCGTA82350.029.04218942-43
CGGCTAT97050.027.70152934-35
CCGGCTA99100.026.79286432-33
ATCGGAA203050.026.2247332
GATCGGA203500.026.1681751
ATCTCGT90150.025.66000442-43
ATGCCGT106050.025.21650948-49
TCACCGG110600.024.32926230-31
TCGGAAG220750.024.2726333
GTATGCC111150.024.18750646-47
ACCGGCT112500.024.12930132-33
GTCACCG112050.023.69662928-29
CCGTCTT119150.022.60362652-53
GCCGTCT120700.022.31332450-51
TATGATC105500.022.15152538-39
CGGAAGA246050.022.0277824
Produced by FastQC (version 0.11.5)