FastQCFastQC Report
Thu 16 May 2019
HFLLHBGXB_n01_ALm217.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLLHBGXB_n01_ALm217.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17136351
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT696400.4063875675749172TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG104700.039.2878644-45
TATGCCG106250.038.9383648-49
CGTATGC106950.038.81634546-47
CTCGTAT103500.037.44871544-45
TCTCGTA111950.032.9887142-43
CGGCTAT134550.031.04772434-35
ATCTCGT117300.030.99818242-43
GTATGCC137050.030.25653346-47
CCGGCTA137050.030.08289132-33
ATGCCGT140350.029.41005148-49
CCGTCTT146550.028.23109252-53
TCACCGG149800.028.1091530-31
GCCGTCT147400.027.87461950-51
ACCGGCT150600.027.69162632-33
TATGATC134900.027.18245538-39
GTCACCG155300.026.67017228-29
TGCCGTC164350.025.38997850-51
CGTCTTC172500.023.88777752-53
GATCTCG154200.023.6572740-41
GCTATGA173650.023.57810236-37
Produced by FastQC (version 0.11.5)