FastQCFastQC Report
Fri 24 May 2019
HFLKTBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLKTBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35571044
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27705967.788908304181345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC293950.048.45523513
ATCGGAA390350.047.541512
GATCGGA393100.047.197191
CGGAAGA382500.046.841774
TCGGAAG391000.046.8263783
CACACGT313500.046.68275512
CGTCTGA295250.046.5832416
GCACACG317450.046.53246711
ACGTCTG298200.046.5092415
CACGTCT303900.046.43181614
AGAGCAC423200.038.0396778
AGCACAC406400.037.71710210
GAACTCC351700.036.93620321
CTGAACT391900.034.8272719
TGAACTC425250.031.86463420
GAGCACA499150.031.8166899
GAAGAGC541950.031.4400426
AACTCCA410050.031.26204522
TCTGAAC446750.030.93544618
CTCCAGT395250.029.49299224
Produced by FastQC (version 0.11.5)