FastQCFastQC Report
Fri 24 May 2019
HFLKTBGXB_n01_mb217.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLKTBGXB_n01_mb217.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30018785
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG50300.038.54846643
CGTATGC55100.036.65125344
CTCGTAT47800.035.65883642
TATGCCG57850.035.39286446
TCTCGTA47700.034.11945741
ATGCCGT60450.033.75445647
ATCTCGT55650.029.1195140
TGCCGTC70800.028.86958948
CCGTCTT74850.027.0735350
GCCGTCT76150.026.19773749
CATCTCG67400.024.04275939
CGTCTTC86300.022.99472451
GTATGCC100900.020.81248945
CGTCTGA157200.015.02835816
GTCGTAT14700.014.76183842
ACGTCTG176150.013.68976915
CGCGTAT6650.013.68414942
GCTTGAA149000.013.50660559
ACACGTC184850.012.9320713
CACGTCT192300.012.57662614
Produced by FastQC (version 0.11.5)