FastQCFastQC Report
Fri 24 May 2019
HFLKTBGXB_n01_mb216.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLKTBGXB_n01_mb216.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27925278
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1404310.5028812962936304TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG202350.061.45511243
CTCGTAT198500.061.11284342
CGTATGC220100.060.7442944
TCTCGTA196450.060.23609541
ATGCCGT217600.059.72062347
TATGCCG225200.059.44613646
ATCTCGT212800.056.66051540
TGCCGTC227450.056.36526548
CGTCTGA311450.055.60387416
ACACGTC314050.055.22164513
ACGTCTG315900.054.99747515
GCCGTCT212750.054.9128849
CCGTCTT219650.054.81294650
CATCTCG226850.054.49312639
CGCGTAT27650.053.54386542
CACGTCT327350.053.1808914
GTATGCC255450.053.1054745
TCGCGTA27100.052.30574841
CGTCTTC223550.051.88403751
ATCGGAA345050.051.8173642
Produced by FastQC (version 0.11.5)