FastQCFastQC Report
Fri 24 May 2019
HFLKTBGXB_n01_mb215.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLKTBGXB_n01_mb215.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33382400
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG53000.041.07493643
CTCGTAT53100.040.7340542
TCTCGTA57050.037.79108441
CGTATGC56950.037.61150444
ATGCCGT59400.036.1776547
TATGCCG60900.035.45914546
TATCTCG60600.034.8261739
CGCCAAT65350.033.47376633
ATCTCGT69400.030.91467340
TGCCGTC74100.028.95360848
ACGCCAA79500.027.7800532
CCGTCTT79650.027.02396850
GCCGTCT84250.025.2576449
GTCACGC92900.023.8105229
CGTCTTC92200.023.61115851
TCACGCC101700.021.85359630
GTATGCC102400.021.49861345
CACGCCA107950.020.58833531
AGTCACG113100.020.17678328
GCCAATA114950.019.54766734
Produced by FastQC (version 0.11.5)