FastQCFastQC Report
Fri 24 May 2019
HFLKTBGXB_n01_mb214.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLKTBGXB_n01_mb214.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32377995
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC5872981.813880075032441TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC1656410.5115851058720591TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC359860.11114338611763948TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG976850.064.9722543
CGTATGC1039650.064.90864644
CTCGTAT771700.064.8833542
TCTCGTA747250.064.7818341
CGTCTGA1266300.064.7695116
ATGCCGT1029400.064.6705747
ACACGTC1276700.064.5765513
TATGCCG1052900.064.5702946
ACGTCTG1277300.064.3406115
TGCCGTC1009800.064.1028148
GATCTCG745900.064.0168239
GCCGTCT951700.063.93780549
CCGTCTT979200.063.9149150
CACGTCT1289250.063.89103314
GATCGGA1318400.063.760541
ATCGGAA1325800.063.705612
ATCTCGT737500.063.69735740
CGTCTTC956000.063.1813251
TCGGAAG1341850.063.05823
CGGAAGA1335450.062.982494
Produced by FastQC (version 0.11.5)