FastQCFastQC Report
Fri 24 May 2019
HFLKTBGXB_n01_mb213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLKTBGXB_n01_mb213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40602682
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG64050.039.0158443
CGTATGC67700.037.0672844
CTCGTAT61300.036.14154442
TCTCGTA61450.035.3699841
TATGCCG72400.034.70932446
ATGCCGT72750.034.30166647
AATCTCG67500.031.42192839
ATCTCGT76100.028.00900740
TGCCGTC91000.027.57635948
CCGTCTT95150.026.07934250
GCCGTCT104150.024.06095749
CGTCTTC110450.022.53004351
GTATGCC124900.020.48399545
GACCAAT153550.015.271811535
ACCAATC152500.013.97702236
GCTTGAA191450.012.81511159
ACTGACC214750.012.38650332
CCAATCT185450.011.96537837
CAATCTC188050.011.76273438
TGCTTGA220400.011.06825858
Produced by FastQC (version 0.11.5)