FastQCFastQC Report
Fri 24 May 2019
HFLKTBGXB_n01_mb212.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLKTBGXB_n01_mb212.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40080662
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC6411241.5995843581625473TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC416150.10382812539373724TruSeq Adapter, Index 2 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT795650.065.74142
TCTCGTA782800.065.6312741
CGATGTA974500.065.61423534
TCGTATG800700.065.5969743
CGTATGC845300.065.4234544
TATCTCG810750.065.34112539
CACCGAT995350.065.2422631
ACCGATG993800.065.1960232
CCGATGT992900.064.9977233
ATGCCGT841100.064.9423947
TATGCCG860800.064.6682646
GTCACCG1015050.064.1961729
ATCTCGT809450.064.16675640
TGCCGTC831550.064.0678948
TCACCGA1016700.064.0405830
GCCGTCT787700.063.7198249
CCGTCTT811050.063.63286250
TCGCGTA63850.063.09276641
CGTCTGA1058000.062.936116
CGTCTTC792100.062.66308651
Produced by FastQC (version 0.11.5)