FastQCFastQC Report
Fri 24 May 2019
HFLKTBGXB_n01_GG020.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLKTBGXB_n01_GG020.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23460210
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC1802830.7684628568968479TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA276800.062.1997031
TCGTATG251850.062.09168243
CTCGTAT242300.062.06904642
CGTATGC254800.061.92197844
ACGGCTA261050.061.56696732
TCTCGTA235350.061.4632141
ATCGGAA281800.061.2238662
CGGCTAC259200.061.1962133
ATGCCGT258600.060.4834647
CGTCTGA275700.060.1608516
TATGCCG262800.059.77050446
ACACGTC281950.059.18712613
CCGTCTT261500.058.92949350
ATCTCGT237350.058.88085640
ACGTCTG282550.058.8627515
TGCCGTC265050.058.7081448
GCCGTCT261950.058.70800849
GTCACGG277200.058.20702729
AGTCACG279600.058.0705428
TCACGGC276800.058.0642930
Produced by FastQC (version 0.11.5)