FastQCFastQC Report
Fri 24 May 2019
HFLKTBGXB_n01_GG019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLKTBGXB_n01_GG019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29014464
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1505740.518961852957201TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT209550.061.06390442
TCTCGTA208750.060.8622141
CGTATGC220800.060.70993844
TCGCGTA31500.060.66660341
TCGTATG203250.060.6657643
TATCTCG232900.060.00606539
TATGCCG224550.059.93028346
ATGCCGT214700.059.6475647
TGCCGTC215200.058.43576448
ATCTCGT227550.058.3716240
TATCGCG41850.057.7893639
CGCGTAT34100.057.58038742
GCCGTCT200500.057.32612249
CCGTCTT211900.057.29769550
CGTCTGA349500.057.08069216
ATCGCGT37750.056.7415640
ACGTCTG356500.056.0676915
CGTCTTC204200.055.51582351
ACACGTC360250.055.46529813
GCTTATC328950.053.8911136
Produced by FastQC (version 0.11.5)