FastQCFastQC Report
Fri 24 May 2019
HFLKTBGXB_n01_GG018.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLKTBGXB_n01_GG018.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30031295
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC6821532.2714738075730665TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT893550.063.3089542
TCGTATG955450.063.2035843
CGTATGC963350.063.03020544
TCTCGTA870700.063.00876641
ATGCCGT957400.062.9138947
CGATCAG969950.062.79731833
TATGCCG962400.062.7506846
GATCTCG836100.062.6691639
ACGATCA976500.062.60200532
TGCCGTC960950.062.49603348
CACGATC977600.062.42047531
CCGTCTT959100.062.22215750
ATCTCGT843400.062.15588440
GCCGTCT960700.062.0531649
TCACGAT987600.062.04369730
CGTCTTC968300.061.61620751
GTCACGA996350.061.4984229
GTATGCC996300.060.75605445
CGTCTGA1037500.060.628416
AGTCACG1024500.060.29374728
Produced by FastQC (version 0.11.5)