FastQCFastQC Report
Fri 24 May 2019
HFLKTBGXB_n01_GG017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLKTBGXB_n01_GG017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26331355
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC998080.37904619796436606TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA22800.059.25370841
AATCTCG166250.058.92552639
TCTCGTA159050.055.69568341
CGCGTAT25200.055.69359242
CTCGTAT159950.055.66654642
CGTCTGA281150.055.42173816
TCGTATG158650.054.84305243
CGTATGC172500.054.4367444
ACGTCTG287950.054.20997615
TATGCCG176150.054.1430646
ATGCCGT165450.053.98496647
ATCTCGT173750.053.21953240
ACACGTC295100.053.0039613
TGCCGTC165600.051.7804148
CACGTCT306800.050.98252514
CCGTCTT165850.049.95042850
ATCGCGT30550.049.8357640
GCCGTCT158650.049.01873449
GCACACG329950.047.4692211
CACACGT330100.047.3412612
Produced by FastQC (version 0.11.5)