FastQCFastQC Report
Fri 24 May 2019
HFLKTBGXB_n01_GG016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLKTBGXB_n01_GG016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21181552
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2184271.031213387951931TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG302250.063.6183743
CTCGTAT287700.063.46615642
CGTATGC313450.063.24294744
ATGCCGT311400.063.05251347
TCTCGTA276550.063.05134641
TATGCCG314550.062.9327746
TGCCGTC312750.061.6948448
CCGTCTT304950.061.37901350
ATCTCGT281400.061.25568840
GCCGTCT301050.061.1627249
CGTCTTC300100.060.7500351
CGTCTGA374850.060.66183516
ACACGTC377550.060.25625613
CATCTCG289700.059.87521739
ACGTCTG381700.059.65571615
ATCGGAA394300.059.314572
GATCGGA392650.059.2791751
GTATGCC342450.058.3267645
CACGTCT392050.058.2150114
TCGGAAG407350.057.551563
Produced by FastQC (version 0.11.5)