FastQCFastQC Report
Fri 24 May 2019
HFLKTBGXB_n01_GG015.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLKTBGXB_n01_GG015.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22344704
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC6028822.698097947504697TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGCTCTCGTATGC307600.13766125521286834TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAGCTCGTATGC278670.12471411570276339TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT774450.065.90008542
TCTCGTA739400.065.8764841
TCGTATG839950.065.7987343
CGATCTC701700.065.6847538
CGTATGC849300.065.64274644
ATGCCGT842350.065.59820647
TATGCCG844750.065.58646
ACGATCT701950.065.57674437
CACGATC707800.065.4604536
TCACGAT767100.065.4235435
GATCTCG706850.065.3200739
ATCACGA807450.065.23624434
TGCCGTC845350.065.1958148
CATCACG856400.065.132433
ATCTCGT710900.065.05176540
CCGTCTT839250.065.0231450
CGTCTGA887550.064.9553316
GCCGTCT839950.064.9357649
ACACGTC892450.064.6967413
CGTCTTC842800.064.6452951
Produced by FastQC (version 0.11.5)