FastQCFastQC Report
Fri 24 May 2019
HFLKTBGXB_n01_GG014.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLKTBGXB_n01_GG014.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39036294
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1212850.31069803911201205TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG187300.059.40392743
CTCGTAT171100.058.9943542
CGTATGC190750.058.98975844
TCTCGTA167950.058.82984541
ATGCCGT193550.057.48502747
TATGCCG197200.056.91826646
AATCTCG173000.056.7075739
TGCCGTC195000.056.48337648
GCCGTCT194900.054.80631649
ATCTCGT182100.053.7395540
CCGTCTT200550.053.66346450
CGTCTTC207650.050.9353651
GTATGCC246050.045.88815745
CGTCTGA309900.040.04808816
GTAATCT259700.038.4094537
ACGTCTG328200.037.94288315
ACACGTC351400.035.39844513
GCTTGAA307250.035.01658659
CACGTCT361900.034.5068114
CCTTGTA347700.034.25474533
Produced by FastQC (version 0.11.5)