FastQCFastQC Report
Sat 22 Jan 2022
HFLJJAFX3_n01_CHAR29_pH4_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLJJAFX3_n01_CHAR29_pH4_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18972759
Sequences flagged as poor quality0
Sequence length149
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCGGCCGTAATCTC1551100.8175405590720886No Hit
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCGGCCGTAATCGC213650.11260881983479577No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)