FastQCFastQC Report
Fri 1 Sep 2023
HFLFKDRX3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLFKDRX3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029609422
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGACCTCAGGTCAGGCGGG192250611.8672188297049208Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGACCTCAGGTCAGGCGGGG78070880.7582572413561305Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA54867250.5328938219448422Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCATCCACGCTTGGTCGTA36746910.35690145422931063Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAGACCTCAGGTCAGGCGGGGC32855800.3191093564021406Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA20828180.20229204934373649Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGAGACCTCAGGTCAGGCGGGG20760910.20163869479430618Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATGCATTGGATGGATGCC17223210.1672790636136972Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGTCGTGCCTCCGGC15587750.15139478783829544Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCTGGAGAAGCGTCCTCAGTGGCGGACCGGGCCCAAGTCCCCTGGAAGG15271950.1483276053392604No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGCCGGGTCGTGCCTCCGG13984340.13582179515058868Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAGACCTCAGGTCAGGCGGGGCT13098790.12722096088199938Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10593330.10288687898196021No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGCGGCGAGAGCGGGTCGCC10576480.10272322469092557Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAATGCATTGGATGGATGC10502830.10200790489658126Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)