FastQCFastQC Report
Fri 1 Sep 2023
HFLFKDRX3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLFKDRX3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029609422
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGTCGG737635967.1642308650124225No Hit
AGCAGAGC678909016.5938500123787716No Hit
ACGACATT677329006.578504290338555No Hit
GCGGATAG639605616.212118851414318No Hit
GCCGAGAT638210746.198571286967107No Hit
CATCTCCA572372355.559121136325421No Hit
TTATCGTT539449925.239364641323183No Hit
ATGCCTTA528461625.132641647484846No Hit
GTATGTCA521553185.065543970905892No Hit
TGTCAGAC516331185.014825709316401No Hit
CGTATCGG457230354.44081357678174No Hit
AGGCCCGA437120194.2454952398444545No Hit
TAATGACC429102254.16762163235138No Hit
GAATCCGC399655583.8816231811833593No Hit
TGGAGGCT390585853.793534146582431No Hit
CCCGATTA373462033.6272204004753172No Hit
GGGGGGGG289619962.812910933132467No Hit
ATTCTAAG287149132.7889131923658717No Hit
GTTTATCT286450182.7821246958247046No Hit
CCAAGATG281929202.7382150354875052No Hit
TACGTGAC279404432.7136934067411826RNA PCR Primer, Index 17 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)