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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2018-11-16, 03:11 based on data in: /beegfs/mk5636/logs/html/HFLF5BGX9/merged


        General Statistics

        Showing 25/25 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        HFLF5BGX9_n01_AKM13
        18.6%
        39%
        18.2
        HFLF5BGX9_n01_AKM14
        22.2%
        41%
        16.3
        HFLF5BGX9_n01_AKM15
        19.1%
        40%
        23.4
        HFLF5BGX9_n01_AKM16
        25.1%
        40%
        19.5
        HFLF5BGX9_n01_AKM17
        21.0%
        40%
        20.5
        HFLF5BGX9_n01_AKM18
        12.4%
        38%
        19.2
        HFLF5BGX9_n01_AKM19
        13.1%
        38%
        17.2
        HFLF5BGX9_n01_AKM20
        14.2%
        38%
        16.4
        HFLF5BGX9_n01_AKM21
        21.0%
        39%
        18.2
        HFLF5BGX9_n01_AKM22
        19.9%
        39%
        16.6
        HFLF5BGX9_n01_AKM23
        26.1%
        39%
        16.8
        HFLF5BGX9_n01_AKM24
        23.7%
        39%
        17.6
        HFLF5BGX9_n01_AKM25
        23.9%
        40%
        18.9
        HFLF5BGX9_n01_AKM26
        13.4%
        38%
        15.3
        HFLF5BGX9_n01_AKM27
        12.2%
        38%
        16.5
        HFLF5BGX9_n01_DJ14
        96.5%
        41%
        16.1
        HFLF5BGX9_n01_DJ15
        94.4%
        43%
        7.9
        HFLF5BGX9_n01_DJ16
        94.6%
        36%
        8.0
        HFLF5BGX9_n01_DJ17
        97.3%
        35%
        24.3
        HFLF5BGX9_n01_DJ18
        94.4%
        41%
        3.3
        HFLF5BGX9_n01_DJ19
        93.1%
        39%
        4.0
        HFLF5BGX9_n01_DJ20
        91.0%
        35%
        3.4
        HFLF5BGX9_n01_DJ21
        96.9%
        46%
        10.8
        HFLF5BGX9_n01_DJ22
        96.0%
        30%
        3.7
        HFLF5BGX9_n01_undetermined
        79.4%
        43%
        28.7

        Demultiplexing Report

        Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.

        Showing 25/25 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        28,703,789
        7.5
        AKM13
        18,194,227
        4.8
        AKM14
        16,313,831
        4.3
        AKM15
        23,420,096
        6.2
        AKM16
        19,494,627
        5.1
        AKM17
        20,522,787
        5.4
        AKM18
        19,162,694
        5.0
        AKM19
        17,229,225
        4.5
        AKM20
        16,432,844
        4.3
        AKM21
        18,222,304
        4.8
        AKM22
        16,560,972
        4.4
        AKM23
        16,806,469
        4.4
        AKM24
        17,557,951
        4.6
        AKM25
        18,899,075
        5.0
        AKM26
        15,285,451
        4.0
        AKM27
        16,476,072
        4.3
        DJ14
        16,077,257
        4.2
        DJ15
        7,884,694
        2.1
        DJ16
        7,985,583
        2.1
        DJ17
        24,291,003
        6.4
        DJ18
        3,300,467
        0.9
        DJ19
        3,966,617
        1.0
        DJ20
        3,407,030
        0.9
        DJ21
        10,753,955
        2.8
        DJ22
        3,665,215
        1.0

        Run Statistics

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined % PhiX Aligned
        4.0
        409,455,856
        380,614,235
        7.5
        5.5

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

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        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (76bp).


        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

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        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

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        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

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