FastQCFastQC Report
Fri 16 Nov 2018
HFLF5BGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLF5BGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28703789
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2312440.8056218640681897No Hit
CTGAACAACATTACCTTTCTGCAAAAAAAAGCAAAAACAATGCGGAACAA1981110.6901911103095135No Hit
GTGTGATGAGACTAGGCAGGGGACATTGCTGGTGCACATTAGGTTATTAA1488990.5187433617213393No Hit
AACTCGGCGACTTTAACGGGCGCCTCGTCGTCGGAAAAATCGCTCAACGC860770.29988027016224234No Hit
CAGCTACGCCAATATCCAAGCTTAATCATACAAAAACTCCATACGTTTAT645030.2247194612530074No Hit
CGGTTTTTTGCATACATCGACGTCAAGAAAACACAATTTATAATAAACAA557210.19412419733157876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACGC238250.067.793
AGCTACG237700.067.550392
CAGCTAC238200.066.603761
GGCAGGG174250.066.0309615
CAGGGGA174150.066.0282217
CTACGCC246250.065.64874
GACTAGG175750.065.5701510
GGGGACA175550.065.4016419
TAACGGG103850.065.3711414
CGAAGTA220500.065.1180270
AGGGTTC173500.065.0486864
CGGGCGC104750.064.94231417
AGACTAG177600.064.9259649
CCAGGTG224950.064.8722762
CTAGGCA178300.064.74721512
GCGGAAC225750.064.6936142
ACTAGGC178200.064.56731411
GGTGACG225250.064.4920365
GTTCGTA171550.064.46290667
AGGTGAC226150.064.45055464
Produced by FastQC (version 0.11.5)