FastQCFastQC Report
Fri 16 Nov 2018
HFLF5BGX9_n01_DJ21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLF5BGX9_n01_DJ21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10753955
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCGGCGACTTTAACGGGCGCCTCGTCGTCGGAAAAATCGCTCAACGC551729851.30482692181621No Hit
CCTGTCGAGACTTGAGCAAGCTTCGGAGCATCTGAAAAATACAGTAAATC8686778.077744420541094No Hit
CCTGTCGAGACTTGAGCACTCCGAATTCAGCGCCGGAACTTTGCAAACAG544750.5065578198904497No Hit
ACTCGGCGACTTTAACGGGCGCCTCGTCGTCGGAAAAATCGCTCAACGCG512600.4766618420850748No Hit
CCTGTCGAGACTTGAGCAAGCTTGTTAAGAAGAAATTCCATCAATTTTAG386610.3595049449249137No Hit
AACTCGGCGACTTTAACGGGCGCCTCGTCGTCGGAAAAATCGCTCACCGC283230.26337287072523546No Hit
CCTGTCGAGACTTGAGCAAGCTTGTCTCCAAGCAACATCAACTGTCTCCG262040.24366849219659187No Hit
CCTGTCGAGACTTGAGCAAGCTTCAGACTCAAGCGCCTCGACGCGAATCA259130.24096251100176633No Hit
CCTGTCGAGACTTGAGCAAGCTTCACGTAGTTACAGAAATAGTACTCCGA209540.19484924383633742No Hit
CCTGTCGAGACTTGAGCAAGCTTTGGAAGTTTCGGTTGTTGCATCTCTAC205740.19131566014549994No Hit
AACTCGGCGCCTTTAACGGGCGCCTCGTCGTCGGAAAAATCGCTCAACGC186690.17360124716906478No Hit
CCTGTCGAGACTTGAGCAAGCTTCTTGTACTCCGAATTCAGCGCCGGAAC177690.16523223316444974No Hit
CCTGTCGAGACTTGAGCAAGCTTTGAGCAGTTACAGAAAACCTACAGTAC159540.14835472158847607No Hit
CCTGTCGAGACTTGAGCAAGCTTGGAATTTGCAACAAAGTTTCTGAAAGA155680.14476534447094117No Hit
CCTGTCGAGACTTGAGCAAGCTTCACCCTTAGAATAAGCTCATTTAGTAT123380.11472988309882272No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG115340.1072535639213666No Hit
CCTGTCGAGACTTGAGCAAGCTTACAACATCCAGGATTCCCAGGCCGTCT107910.1003444779153344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTCG4641800.069.9381561
AACTCGG5815950.069.844271
CTGTCGA4659550.069.754752
TGTCGAG4669050.069.639063
CGAGACT4676550.069.5436
TCGAGAC4677900.069.51325
AGACTTG4678050.069.5070958
GTCGAGA4677050.069.5018844
GAGACTT4682050.069.476127
ACTTGAG4679650.069.46709410
GACTTGA4682050.069.451879
GCAAGCT4605850.069.4490716
AGCAAGC4607500.069.4436315
GAGCAAG4613400.069.3438814
CAAGCTT4608300.069.30302417
AGCTTCG1035800.069.2466519
TGAGCAA4626150.069.2030113
ACTCGGC5880150.069.183432
GCATCTG902150.069.1634228
CTTGAGC4703650.069.144411
Produced by FastQC (version 0.11.5)