FastQCFastQC Report
Fri 16 Nov 2018
HFLF5BGX9_n01_DJ20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLF5BGX9_n01_DJ20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3407030
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGCTAGAGCAATTTTTGCCGACTATTAAAATGATCTACACAAAATTTG134993939.62216358529276No Hit
TTAAAATTGGGTTACGGAAGCTTCTTGTACTGCGCTGGAATTTGAGAGAG204530.6003175786535487No Hit
TTAAAATTGGGTTACGGAAGCTTCACGTAGTTACAGAAATAGTACTGCGC194170.569909862842417No Hit
AGGCTAGAGCAATTTTTGCCGACTATTAAAATGATCTACACAAAATTTGC160720.47173051015107No Hit
TTAAAATTGGGTTACGGAAGCTTGTCTCCAAGCAACATCAACTGTCTCCG128360.3767504248568401No Hit
TTAAAATTGGGTTACGGAAGCTTGTACTGCGCTGGAATTTGAGAGAGAGA114610.3363926939299038No Hit
GGCTAGAGCAATTTTTGCCGACTATTAAAATGATCTACACAAAATTTGCA94440.27719157154471785No Hit
TTAAAATTGGGTTACGGAAGCTTTGAGCAGTTACAGAAAACCTACAGTAC91790.2694135361297083No Hit
TTAAAATTGGGTTACGGAAGCTTCAGACTCAAGCGCCTCGACGCGAATCA90200.26474671488070256No Hit
TTAAAATTGGGTTACGGAAGCTTTGGAAGTTTCGGTTGTTGCATCTCTAC81080.23797853262225457No Hit
TTAAAATTGGGTTACGGAAGCTTACAACATCCAGGATTCCCAGGCCGTCT76700.2251227608797105No Hit
TTAAAATTGGGTTACGGAAGCTTTTCGCCACCGATGATACAAGGCGTTCG57630.16915025696867947No Hit
TAAAATTGGGTTACGGAAGCTTCTTGTACTGCGCTGGAATTTGAGAGAGA55080.16166573232404763No Hit
TAAAATTGGGTTACGGAAGCTTCACGTAGTTACAGAAATAGTACTGCGCT51220.15033621658746768No Hit
GCGGCTAGAGCAATTTTTGCCGACTATTAAAATGATCTACACAAAATTTG46540.13659991253379042No Hit
TTAAAATTGGGTTACGGAAGCTTTTGCCCTTTTGCTCTACGAGAGGTTTC41100.12063292662524251No Hit
TTAAAATTGGGTTACGGAAGCTTGAGTGTTGTCATAGCACAATTGCACGT39150.11490946660287699No Hit
TTAAAATTGGGTTACGGAAGCTTCACCCTTAGAATAAGCTCATTTAGTAT38720.1136473702902528No Hit
TAAAATTGGGTTACGGAAGCTTGTCTCCAAGCAACATCAACTGTCTCCGG36940.1084228785775294No Hit
TTAAAATTGGGTTACGGAAGCTTTACGTAGTTACAGAAATAGTACTGCGC34080.10002847054472663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGCTA1402100.069.8691
AGGCTAG1419350.069.052792
GGCTAGA1436650.068.624653
GCTAGAG1437750.068.547384
CTAGAGC1439250.068.456495
CGACTAT1428050.068.4291321
TGCCGAC1435250.068.3955318
TAGAGCA1440200.068.389466
CCGACTA1430100.068.3726420
GCCGACT1431600.068.37189519
GACTATT1430000.068.3392722
TCTACAC1427450.068.3153536
ACTATTA1428950.068.3096623
GATCTAC1428650.068.30363534
TAACAGC1417800.068.3023366
CTACACA1427550.068.30076637
AGTTAAC1418550.068.2913563
GTTAACA1417100.068.2896164
GAGCAAT1443200.068.2768258
CTATTAA1432400.068.2668624
Produced by FastQC (version 0.11.5)