FastQCFastQC Report
Fri 16 Nov 2018
HFLF5BGX9_n01_DJ19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLF5BGX9_n01_DJ19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3966617
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGTGGACGTCCGAAGAAAGTGAAAGAAAATTTGAAGAAAAGAATTCAG128486132.39185936025585No Hit
ATATTATTCGATTGCTGAAGCTTCATTGATTTCCTTCCAAATAATCTGTG2090485.270183635072406No Hit
ATATTATTCGATTGCTGAAGCTTTTTCGCGAAGAGCGTGTGCTAATTTGT1521903.836770729314174No Hit
ACGTGGACGTCCGAAGAAAGTGAAAGAAAATTTGAAGAAAAGAATTCAGC386360.9740290025480152No Hit
ATATTATTCGATTGCTGAAGCTTGATACTTTCTTCGGACGTCCACGTGAG329870.8316154546809031No Hit
GTATTATTCGATTGCTGAAGCTTCATTGATTTCCTTCCAAATAATCTGTG326210.8223884483931774No Hit
CACGTGGACGTCCGAAGAAAGTATCAAGCTTCAGCAATCGAATAATATAG240490.6062849022227254No Hit
ATATTATTCGATTGCTGAAGCTTTCTTCGGACGTCCACGTGAGATCGGAA236640.596578898340828No Hit
CACGTGGACGTCCGAAGAAAGCTTCAGCAATCGAATAATATAGATCGGAA145960.3679709939225289No Hit
ATATTATTCGATTGCTGAAGCTTCAAAAAATAAATTAACTTACCCTGTAA145950.3679457835228357No Hit
ATATTATTCGATTGCTGAAGCTTGTCTCCAAGCAACATCAACTGTCTCCG137210.3459118941909441No Hit
ATATTATTCGATTGCTGAAGCTTACAACATCCAGGATTCCCAGGCCGTCT110740.27917996620293817No Hit
ATATTATTCGATTGCTGAAGCTTCACGTAGTTACAGAAATAGTACAACTT110070.2774908694234911No Hit
ATATTATTCGATTGCTGAAGCTTTACCATGAAGAGTTTATCAGAGTCCGA104230.26276799600263906No Hit
ATATTATTCGATTGCTGAAGCTTCAGACTCAAGCGCCTCGACGCGAATCA98800.2490787489692098No Hit
CACGTGGACGTCCGAAGAAAGTTGAAGCTTCAGCAATCGAATAATATAGA83890.2114900430265892No Hit
ATATTATTCGATTGCTGAAGCTTGTCTCCCGGCTCAACGAATCCAAAGAC82170.20715385427935187No Hit
ATATTATTCGATTGCTGAAGCTTTTCGCCACCGATGATACAAGGCGTTCG79220.19971678636984616No Hit
ATATTATTCGATTGCTGAAGCTTCAACTTTCTTCGGACGTCCACGTGAGA77240.19472512723058466No Hit
ATATTATTCGATTGCTGAAGCTTCCACTTTCTTCGGACGTCCACGTGAGA72920.18383423456310505No Hit
CACGTGGACGTCCGAAGAAAGTGGAAGCTTCAGCAATCGAATAATATAGA69370.17488454267200487No Hit
ATATTATTCGATTGCTGAAGCTTTGGAAGTTTCGGTTGTTGCATCTCTAC57870.14589258302477903No Hit
ATATTATTCGATTGCTGAAGCTTGGAATTTGCAACAAAGTTTCTGAAAGA56080.1413799214796891No Hit
ATATTATTCGATTGCTGAAGCTTCACCCTTAGAATAAGCTCATTTAGTAT55360.13956477270177584No Hit
CACGTGGCCGTCCGAAGAAAGTGAAAGAAAATTTGAAGAAAAGAATTCAG51420.12963187522263936No Hit
CACGTGGACGTCCGAAGAAAGTGTAAGCTTCAGCAATCGAATAATATAGA48650.1226485945076119No Hit
ATATTATTCGATTGCTGAAGCTTACACTTTCTTCGGACGTCCACGTGAGA48620.12257296330853218No Hit
ATATTATTCGATTGCTGAAGCTTTGAGCAGTTACAGAAAACCTACAGTAC48340.12186707211712146No Hit
ATATTATTCGATTGCTGAAGCTTCTTGTACAACTTCTGGACCAACTTCCG47780.12045528973430003No Hit
CGTGGACGTCCGAAGAAAGTGAAAGAAAATTTGAAGAAAAGAATTCAGCC46460.11712751697479237No Hit
CACGTGGACGTCCGAAGAAAGTGAAAGAAAATTTGAAGAAAAGAATTCCG45100.11369890261651175No Hit
ATATTATTCGATTGCTGAAGCTTCTGGCACCTATTGGGATGTTGTTGGTA44840.11304343222448751No Hit
GTGGACGTCCGAAGAAAGTGAAAGAAAATTTGAAGAAAAGAATTCAGCCG42990.10837950828123813No Hit
TATTATTCGATTGCTGAAGCTTCATTGATTTCCTTCCAAATAATCTGTGT42460.10704335709749643No Hit
ATATTATTCGATTGCTGAAGCTTTCATGACGGATCCGGAGAAGTTTATGC41610.10490047312357105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATTAT2236900.069.621031
CACGTGG1435650.069.3354951
TATTATT2330250.068.29892
ATTATTC2340050.067.991923
TTATTCG2347950.067.7435764
TATTCGA2356350.067.506545
ATTCGAT2358700.067.4146966
CGATTGC2359000.067.397649
TCGATTG2361200.067.342258
GATTGCT2362250.067.32883510
CTGAAGC2357750.067.3063515
GCTGAAG2357200.067.30572514
ATTGCTG2363150.067.2983111
TTGCTGA2362900.067.15880612
TTCGATT2369050.067.119957
TGCTGAA2364650.067.10847513
CGCGAAG165450.067.06357627
AGCGTGT165100.066.9962734
AGAGCGT165350.066.9356232
GTGCTAA165850.066.90266439
Produced by FastQC (version 0.11.5)