FastQCFastQC Report
Fri 16 Nov 2018
HFLF5BGX9_n01_DJ18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLF5BGX9_n01_DJ18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3300467
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAA54519816.518813852706298No Hit
CCATTTTTATGATTGAGTGGATCTTGAGTAGGTGCTCTCAACGAGTTCCT40319112.216180316300692No Hit
CCATTTTTATGATTGAGTGGCAAAATGGACCTGATTTGAACCAGATCGGA33093810.027005269254321No Hit
GGTTCAAATCAGGTCCATTTTGCCACTCAATCATAAAAATGGAGATCGGA2849388.633263110947633No Hit
CCATTTTTATGATTGAGTGGATCAAGCAAAATGGACCTGATTTGAACCAG1823335.524460629359421No Hit
GGTTCAAATCAGGTCCATTTTGCTTGATCCACTCAATCATAAAAATGGAG1532784.644130663933317No Hit
CCATTTTTATGATTGAGTGGATCAGCAAAATGGACCTGATTTGAACCAGA953352.888530623090611No Hit
CCATTTTTATGATTGAGTGGATGCAAAATGGACCTGATTTGAACCAGATC942622.856020072310979No Hit
GGTTCAAATCAGGTCCATTTTGCATCCACTCAATCATAAAAATGGAGATC883552.6770453999388573No Hit
GGTTCAAATCAGGTCCATTTTGCTGATCCACTCAATCATAAAAATGGAGA805612.4408970003335893No Hit
GGTTCAAATCAGGTCCATTTTGCCACTCAATCATAAAAATGGCCATTTTT384231.164168585839519No Hit
CCATTTTTATGATTGAGTGGATCGCAAAATGGACCTGATTTGAACCAGAT229940.6966892866979127No Hit
GGTTCAAATCAGGTCCATTTTGCGATCCACTCAATCATAAAAATGGAGAT195500.5923404172803425No Hit
CCATTTTTATGATTGAGTGGATGGCAAAATGGACCTGATTTGAACCAGAT187240.5673136559159658No Hit
GGGTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAA182790.5538307154714772No Hit
GGTTCAAATCAGGTCCATTTTGCCATCCACTCAATCATAAAAATGGAGAT182330.5524369733131705No Hit
CCATTTTTATGATTGAGTGGATCATAGCAAAATGGACCTGATTTGAACCA171230.5188053690583787No Hit
GGTTCAAATCAGGTCCATTTTGCTTGATCCACTCAATCATAAAAATGGCC170630.5169874445040656No Hit
GGTTCAAATCAGGTCCATTTTGCTCCACTCAATCATAAAAATGGAGATCG137040.41521396820510553No Hit
GGTTCAAATCAGGTCCATTTTGCTATGATCCACTCAATCATAAAAATGGA134470.4074271913641312No Hit
GGTTCAAATCAGGTCCATTTTGCATCCACTCAATCATAAAAATGGCCATT106110.32149995743026666No Hit
CCATTTTTATGATTGAGTGGAGCAAAATGGACCTGATTTGAACCAGATCG104720.3172884322127747No Hit
GGTTCAAATCAGGTCCATTTTGCTGATCCACTCAATCATAAAAATGGCCA96180.2914133060563854No Hit
CATTTTTATGATTGAGTGGATCTTGAGTAGGTGCTCTCAACGAGTTCCTC94550.28647461101716815No Hit
GGTTCAAATCAGGTCCATTTTGCCACTCAATCATAAAAATGGGATCGGAA83970.25441854137611436No Hit
GGTTCAAATCAGGTCCATTTTGCCACTCAATCATAAAAATGGAGGTTCAA79250.24011753488218487No Hit
GGTTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAC78430.23763303799129032No Hit
AAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACCGGG78140.23675437445670566No Hit
CCATTTTTATGATTGAGTGGATCTTATCATGCAAAATGGACCTGATTTGA73780.22354412269536403No Hit
CCATTTTTATGATTGAGTGGCAAAATGGACCTGATTTGAACCTCCATTTT73570.22290784910135442No Hit
ATTTTTATGATTGAGTGGATCTTGAGTAGGTGCTCTCAACGAGTTCCTCA73150.22163530191333528No Hit
GGTTCAAATCAGGTCCATTTTGCATGATAAGATCCACTCAATCATAAAAA67730.20521338343937387No Hit
GGTTCAAATCAGGTCCATTTTGCGCGTTTGGACCAAGACCCATTCCAGGC67250.2037590437959234No Hit
TTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACC64630.19582077324208969No Hit
AATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACCGGGG64180.19445732982635489No Hit
CCATTTTTATGATTGAGTGGATCAGGGCCTGCAGGAATGGGACAACCAAT63560.19257880778689804No Hit
CCATTTTTATGATTGAGTGGATCAATTTTCGCTTTTCATTGGAAAGAAAA53310.1615225966507164No Hit
GGTTCAAATCAGGTCCATTTTGCTGGTTGAGCGTCTTGTCGGGGGAGCTG52190.15812913748266533No Hit
CAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACCGG51850.15709898023522126No Hit
GGTTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCACA44350.13437492330630785No Hit
CCATTTTTATGATTGAGTGGATCAACTAAAAGTCGAAGTTGAAGATAGTA43740.1325267000094229No Hit
GGTTCAAATCAGGTCCATTTTGCTTGATCCACTCAATCATAAAAATGGGA41780.12658814646533356No Hit
ATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAACCGGGGA39350.11922555202036561No Hit
CCATTTTTATGATTGAGTGGATCAAGCAAAATGGACCTGATTTGAACCTC39120.11852868094121226No Hit
CCATTTTTATGATTGAGTGGCAAAATGGACCTGATTTGAACCAGGTTCAA37990.11510492303058929No Hit
CCATTTTTATGATTGAGTGGCAAAATGGACCTGATTTGAACCTGGTTCAA37770.11443835069400786No Hit
GGGGCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAA35060.10622739145702714No Hit
GTTCAAATCAGGTCCATTTTGCAATTGAATTTCGTAGCAAAAATCCAAAC34860.10562141660558945No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCG207.9264086E-570.0078541
GTGCCCT301.02441845E-770.0046632
GTCAGCG150.002224819570.0036133
CGTACCA207.9287885E-570.0036134
AGGGCAT252.8413033E-670.003634
CGGAACG207.929383E-570.0025545
GGCGGTC301.02461854E-770.0025543
CCCCGGG1200.070.0014849
GCGGGTC207.930573E-570.0004365
CGTCCTA150.002225220770.0004347
GGGTCAA27900.068.246211
GGTTCAA1571650.067.290011
GTTCAAA1584550.066.7291262
TCAAATC1623750.066.1367344
TTCAAAT1601700.066.010453
CCCGGGA1700.065.8857451
ACCGCGG850.065.8847450
CAAATCA1637850.065.781075
CTTGATC200900.065.5897523
GCCACTC407350.065.355722
Produced by FastQC (version 0.11.5)