FastQCFastQC Report
Fri 16 Nov 2018
HFLF5BGX9_n01_DJ17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLF5BGX9_n01_DJ17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24291003
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAACAACATTACCTTTCTGCAAAAAAAAGCAAAAACAATGCGGAACAA1118359346.04006265200329No Hit
CAGCTACGCCAATATCCAAGCTTAATCATACAAAAACTCCATACGTTTAT355777014.6464516100879No Hit
CTGAACAACATTACCTTTCTGCAAAAAAAGCAAAAACAATGCGGAACAAA984240.40518705629405255No Hit
TGAACAACATTACCTTTCTGCAAAAAAAAGCAAAAACAATGCGGAACAAA963530.396661265901618No Hit
CTGAACAACATTACCTTTCTGCAAAAAAAAAGCAAAAACAATGCGGAACA800370.32949236390115305No Hit
CAGCTACGCCAATATCCAAGCTTCCACAATTTGTGGAGCAGCAGTGGCTG793340.32659828826335413No Hit
GAACAACATTACCTTTCTGCAAAAAAAAGCAAAAACAATGCGGAACAAAA656870.27041699348520104No Hit
CAGCTACGCCAATATCCAAGCTTGTCTCCAAGCAACATCAACTGTCTCCG490440.20190191405435173No Hit
CAGCTACGCCAATATCCAAGCTTCAGACTCAAGCGCCTCGACGCGAATCA387580.15955701788024151No Hit
CAGCTACGCCAATATCCAAGCTTTGGAAGTTTCGGTTGTTGCATCTCTAC350150.14414802056547438No Hit
AGCTACGCCAATATCCAAGCTTAATCATACAAAAACTCCATACGTTTATA271710.11185622923845509No Hit
CAGCTACGCCAATATCCAAGCTTGAGTGTTGTCATAGCACAATTGCACGT250840.10326457083719434No Hit
CAGCTACGCCAATATCCAAGCTTGGAATTTGCAACAAAGTTTCTGAAAGA248600.10234241871362826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTAC11819050.069.927271
CTGAACA11825150.069.759481
AGCTACG11913250.069.383582
GCTACGC11987600.069.028873
TACGCCA12003000.068.93565
TGAACAA11982950.068.91022
CGCCAAT12012350.068.905437
ACGCCAA12010100.068.9024356
CTACGCC12012250.068.877854
GCCAATA12021650.068.844278
CCAAGCT11810700.068.8221416
ATATCCA12027650.068.8022612
CCAATAT12033250.068.7988369
CAAGCTT11813900.068.772417
CCATACG3696950.068.7594839
AATATCC12038300.068.7582611
GAACAAC12017600.068.7482153
TATCCAA11872850.068.7414413
CATTACC12049100.068.687429
CATACGT3702400.068.67068540
Produced by FastQC (version 0.11.5)