FastQCFastQC Report
Fri 16 Nov 2018
HFLF5BGX9_n01_DJ16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLF5BGX9_n01_DJ16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7985583
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTTTTTGCATACATCGACGTCAAGAAAACACAATTTATAATAAACAA320712840.161475999936386No Hit
TTTTGGCCGCTAGCACGAAGCTTGGCATATGCGGCAAGTTGTGAACGTTT5196976.507940622494313No Hit
TTTTGGCCGCTAGCACGAAGCTTCTTGTACTGAAATTAAAAAGAAAATTA524240.656483064542689No Hit
TTTTGGCCGCTAGCACGAAGCTTCACGTAGTTACAGAAATAGTACTGAAA493270.6177006738268201No Hit
GGTTTTTTGCATACATCGACGTCAAGAAAACACAATTTATAATAAACAAT306600.38394191131693206No Hit
TTTTGGCCGCTAGCACGAAGCTTTGAGCAGTTACAGAAAACCTACAGTAC235270.29461843925484216No Hit
CGGTTTTTTGCATACATTCGAGTAAATTAATTTTCTTTTTAATTTCAGTA229110.2869045378402554No Hit
TTTTGGCCGCTAGCACGAAGCTTGCTTGATCTTGACTTTCAGTACTGAAA209020.26174670027222807No Hit
TTTTGGCCGCTAGCACGAAGCTTACAACATCCAGGATTCCCAGGCCGTCT179440.22470494640153388No Hit
TTTTGGCCGCTAGCACGAAGCTTGTACTGAAATTAAAAAGAAAATTAATT175220.21942042302985268No Hit
TTTTGGCCGCTAGCACGAAGCTTGTCTCCAAGCAACATCAACTGTCTCCG172890.21650266486491967No Hit
TTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG161750.20255252496905987No Hit
TTTTGGCCGCTAGCACGAAGCTTTTCGCCACCGATGATACAAGGCGTTCG140280.17566657312308945No Hit
GGGTTTTTTGCATACATCGACGTCAAGAAAACACAATTTATAATAAACAA121400.15202396619007028No Hit
CGGTTTTTTGCAACATTCGAGTAAATTAATTTTCTTTTTAATTTCAGTAC114660.14358375587605812No Hit
TTTTGGCCGCTAGCACGAAGCTTCTTCGCATATGTACTGAAATTAAAAAG109570.1372097691552389No Hit
TTTTGGCCGCTAGCACGAAGCTTCAGACTCAAGCGCCTCGACGCGAATCA109300.13687165983998914No Hit
TTTTGGCCGCTAGCACGAAGCTTGGAATTTGCAACAAAGTTTCTGAAAGA100130.1253884656887293No Hit
CGGTTTTTTGCATACATCGAGCTAAAAAAAAAGGAAAGTAATTTGAAAAC98570.12343494520061966No Hit
TTTTGGCCGCTAGCACGAAGCTTTTGCCCTTTTGCTCTACGAGAGGTTTC96910.1213561990401953No Hit
GGGGTTTTTGCATACATCGACGTCAAGAAAACACAATTTATAATAAACAA96820.12124349593511205No Hit
GTTTGGCCGCTAGCACGAAGCTTGGCATATGCGGCAAGTTGTGAACGTTT94480.11831321520294762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTTT3403900.069.75921
TTTTGGC4175600.069.441651
ATCGACG3405250.069.0456816
CGACGTC3401250.069.0441318
CATCGAC3406650.069.03168515
TACATCG3432150.069.0222513
TCGACGT3406500.069.0203417
ACGTCAA3398150.069.0070420
TTTGGCC4239400.068.981732
CGTCAAG3406300.068.9803721
GACGTCA3403850.068.94820419
GTCAAGA3405450.068.9461522
GCATACA3461600.068.9340310
ATACATC3436700.068.9308712
ACATCGA3427000.068.9266814
TGCATAC3463700.068.9205259
ATGAGAG3371300.068.9102366
ATCAGGT3371800.068.8874757
AGAGTTC3364850.068.8863169
GGTTTAT3372850.068.86127561
Produced by FastQC (version 0.11.5)