FastQCFastQC Report
Fri 16 Nov 2018
HFLF5BGX9_n01_DJ15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLF5BGX9_n01_DJ15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7884694
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGATTCTCGATGCAAGAAGCTTACAGAGTGGTTTCAAAAACTGGACCAA5979477.583642434316411No Hit
CAGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCA5209306.6068511980300055No Hit
AGGATTCTCGATGCAAGAAGCTTTTCGAGGAGGGAGAATACACTCACCCA5022046.369353078255162No Hit
CAGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAT4919186.23889779362395No Hit
AGGATTCTCGATGCAAGAAGCTTAGTTTGTTGTCCGCACCACAATGTTCT2971703.7689477866864585No Hit
CAGAACATTGTGGTGCGGACAACAAACTAAGCTTCTTGCATCGAGAATCC2416803.0651791940181825No Hit
CAGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTT2151482.7286791345358488No Hit
CAGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAA1866032.366648597903736No Hit
CAGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTG1549091.96467992289872No Hit
CAGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCT1535061.9468859539761467No Hit
CAGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTC1327921.6841744270608348No Hit
CAGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTA1304371.6543064321836713No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1182061.4991831008280092No Hit
CAGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAC811891.0297038794403435No Hit
CAGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAG742820.9421037772677037No Hit
CAGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCG712320.9034212361316749No Hit
CAGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCC655130.8308883008015275No Hit
AGGATTCTCGATGCAAGAAGCTTCTTCGTTGCCCGAGCGACGAACAGCTT608490.7717357198643346No Hit
CAGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGA360390.4570754426233916No Hit
AGGATTCTCGATGCAAGAAGCTTGTCTCCAAGCAACATCAACTGTCTCCG348200.4416151089693525No Hit
AGGATTCTCGATGCAAGAAGCTTGGAAAAAAAGTTGTCCAGGGATAAAAA336960.427359641350698No Hit
CAGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGT287480.3646051451077239No Hit
CAGAACATTGTGGTGCGGACAACACGTTCCGGCAGGAAAAACAAGAAAAT272350.3454160681441791No Hit
CAGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGG216720.2748616496721369No Hit
CAGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGC198480.2517282217927544No Hit
AGGATTCTCGATGCAAGAAGCTAGTTTGTTGTCCGCACCACAATGTTCTG197300.25023165134880315No Hit
AGGATTCTCGATGCAAGAAGCTTCACGTAGTTACAGAAATAGTACAAGGC197100.24997799534135376No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG187400.2376756789800593No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCAT184140.2335410860586346No Hit
CAGAACATTGTGGTGCGGACAACAAACTAGCTTCTTGCATCGAGAATCCT179030.22706017506830323No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACATC158370.20085750949878334No Hit
AGGATTCTCGATGCAAGAAGCTTTTCGCCACCGATGATACAAGGCGTTCG156540.19853655703062162No Hit
AGGATTCTCGATGCAAGAAGCTTTGAGCAGTTACAGAAAACCTACAGTAC150800.19125662961682469No Hit
AGGATTCTCGATGCAAGAAGCTAGGATGGGAATGCCTACGTGCCTGCTTT135560.17192804184918273No Hit
AGGATTCTCGATGCAAGAAGCTTGAACCATTTGCCAAACTTTTGTTTAAG132340.1678441801292479No Hit
AGGATTCTCGATGCAAGAAGCTTGTACAAGGCTGCTATGAACTTTGATGT130020.16490177044283522No Hit
CAGAACATTGTGGTGCGGACAACAGCAATGTGAGTTTGATGGCGAATACT129180.16383641521154785No Hit
CAGAACATTGTGGTGCGGACAACTAGTAAAGAAGAAGATGATGACGCTGA119280.15128044284280404No Hit
AGAACATTGTGGTGCGGACAACAAACTAAGCTTCTTGCATCGAGAATCCT106570.1351606035693966No Hit
AACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCATTC82700.10488675908031433No Hit
AGGATTCTCGATGCAAGAAGCTTGTTTGTTGTCCGCACCACAATGTTCTG80210.10172874178756969No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATTC4000400.069.591011
CAGAACA3174850.069.184451
GGATTCT4041550.068.911422
GATTCTC4060000.068.551723
AGAGTGG642200.068.0850726
CACCCAG525350.068.075545
ATTCTCG4088400.068.064854
ACAGAGT644650.068.0228924
GAGTGGT643450.067.9632527
CAGAGTG644100.067.95228625
CTCGATG4095550.067.9451457
ATGCAAG4094200.067.9448911
GGAGGGA528000.067.9237529
CGATGCA4094650.067.900699
TGCAAGA4097100.067.8647612
GATGCAA4099400.067.8604310
TCGATGC4100300.067.8596048
TTCTCGA4103200.067.85735
TCTCGAT4104050.067.8534856
GCAAGAA4086950.067.82178513
Produced by FastQC (version 0.11.5)