FastQCFastQC Report
Fri 16 Nov 2018
HFLF5BGX9_n01_DJ14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFLF5BGX9_n01_DJ14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16077257
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGATGAGACTAGGCAGGGGACATTGCTGGTGCACATTAGGTTATTAA868841854.04166892399618No Hit
GAACAAATGTCGTTGTAAAGCTTTAGTAAAAGCATTAGATTTCTGAAATG7230134.497116641228041No Hit
TGTGATGAGACTAGGCAGGGGACATTGCTGGTGCACATTAGGTTATTAAA812930.5056397369277608No Hit
GTGATGAGACTAGGCAGGGGACATTGCTGGTGCACATTAGGTTATTAAAA632120.39317652258715524No Hit
GAACAAATGTCGTTGTAAAGCTTGTCTCCAAGCAACATCAACTGTCTCCG482040.2998272653102454No Hit
GGGTGATGAGACTAGGCAGGGGACATTGCTGGTGCACATTAGGTTATTAA329430.20490435650807848No Hit
GAACAAATGTCGTTGTAAAGCTTACAACATCCAGGATTCCCAGGCCGTCT281860.17531597585334363No Hit
GAACAAATGTCGTTGTAAAGCTTCAGACTCAAGCGCCTCGACGCGAATCA274410.17068210080861432No Hit
GAACAAATGTCGTTGTAAAGCTTTGGAAGTTTCGGTTGTTGCATCTCTAC257950.1604440359446888No Hit
GAACAAATGTCGTTGTAAAGCTTCACGTAGTTACAGAAATAGTACGAACC217290.13515365214352174No Hit
GAACAAATGTCGTTGTAAAGCTTTTGCCCTTTTGCTCTACGAGAGGTTTC211420.1315025318062652No Hit
GAACAAATGTCGTTGTAAAGCTTGGAATTTGCAACAAAGTTTCTGAAAGA201740.12548160423136856No Hit
GAACAAATGTCGTTGTAAAGCTTTTCGCCACCGATGATACAAGGCGTTCG194140.12075442968909435No Hit
GAACAAATGTCGTTGTAAAGCTTTTCAAAAACATTTTAAAATCTTTCACT193530.12037501173241183No Hit
GAACAAATGTCGTTGTAAAGCTTGAGTGTTGTCATAGCACAATTGCACGT187070.11635691337147872No Hit
GAACAAATGTCGTTGTAAAGCTTTGAGCAGTTACAGAAAACCTACAGTAC179040.11136228026957583No Hit
GAACAAATGTCGTTGTAAAGCTTCTTGTACGAACCCTTCTACTTCCGATT179000.11133740040356387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGAT8982300.069.88581
GAACAAA6450200.069.359211
TGTGATG9076900.069.201772
GTGATGA9187750.068.640693
CAGGGGA9170950.068.5720817
AGGGGAC9166200.068.5684718
GCAGGGG9181100.068.56608616
ACTAGGC9192450.068.5654411
GGCAGGG9186700.068.5597115
GACTAGG9196450.068.55237610
CTAGGCA9200400.068.5476512
GGGGACA9167850.068.5399219
GAGACTA9199200.068.537168
GGTGCAC9137550.068.53107531
AGACTAG9199600.068.539
CTGGTGC9138900.068.52759629
GGGACAT9169900.068.5240920
GCTGGTG9142900.068.5070228
TGCTGGT9140250.068.5032227
ATGAGAC9203550.068.502036
Produced by FastQC (version 0.11.5)